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mouse ifnβ duoset elisa kit  (R&D Systems)


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    R&D Systems mouse ifnβ duoset elisa kit
    Mouse Ifnβ Duoset Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+ifn+beta+duoset+elisa+kit/Mouse+IFN-beta+DuoSet+ELISA/pm41803437-218-27-32
    Average 96 stars, based on 135 article reviews
    mouse ifnβ duoset elisa kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: MyD88 signalling in B cells and antibody responses during Oropouche virus-induced neurological disease in mice.
    Article Snippet: Plates were coated with 10 5 FFU of OROV in 100 mM carbonate-bicarbonate buffer (pH 9.6) overnight at 4 ◦ C. After washing five times with washing buffer (PBS containing 0.05% Tween-20), plates were incubated with blocking buffer (washing buffer containing 10% skimmed milk powder) for 30 min at 37 ◦ C. Serum samples were diluted 10-, 50-, 100-, and 200-fold in blocking buffer and added to the plates for 2 h at 37 ◦ C. For the avidity assay, paired samples were incubated with 6 M urea for 10 min at 37 ◦ C. After washing five times with washing buffer, goat anti-mouse peroxidase-conjugated IgM (Abcam #ab97230, RRID:AB_10688258) or goat anti-mouse peroxidase-conjugated IgG (Abcam #ab6789, RRID: AB_955439), respectively diluted 1:5000 or 1:10,000 in blocking buffer, was added for 1 h at 37 ◦ C. Plates were then washed again five times with washing buffer and incubated with 3,3’,5,5’-tetramethylbenzidine (Sigma– Aldrich) for 30 min at room temperature in a dark chamber before reading at 650 nm using an Epoch Spectrophotometer (BioTek). .. IFN-α was quantified using a VeriKine Mouse Interferon-Alpha ELISA Kit (PBL Assay Science), and IFN-β was quantified using a Mouse IFN-Beta DuoSet ELISA Kit (R&D Systems). .. Levels of IFN-γ, IL-1β, IL-6, IL-10, IL-12, CXCL1, GM-CSF, and TNFα were determined using a Mouse Magnetic Luminex Assay Kit (R&D Systems).

    Article Title: T-cell derived extracellular vesicles prime macrophages for improved STING based cancer immunotherapy.
    Article Snippet: The concentration was determined from a standard curve made with IFN-α (IFNa2 PBL Assay Science) ranging from 2 to 500 U/mL. . Enzyme-linked Immunosorbent assay (ELISA) Protein levels of the cytokines CXCL-10 and IL-6 in the cell culture supernatants were determined using the following ELISA kits: CXCL-10 and IL-6 (R&D System) according to manufacturer’s instructions. .. IFN-beta in the cell culture supernatants was determined using the mouse IFN-beta DuoSet ELISA kit (R&D System) according to manufacturer’s instructions. .. IFNγ in the T-EVs were determined using the Human IFNγDuoSet ELISA kit (R&D System) according to manufacturer’s instruction.

    Article Title: TLR9 Monotherapy in Immune-Competent Mice Suppresses Orthotopic Prostate Tumor Development
    Article Snippet: .. IFN-β levels in cell-culture media were measured using the Mouse IFN-beta DuoSet ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. .. GraphPad Prism 8.0 ( Boston, MA, USA) was used to perform unpaired, two-sided t -tests, or one- or two-way ANOVA analyses with post hoc tests for multiple comparisons (outlined in the figure legends).

    Article Title: Targeting STING oligomerization with licochalcone D ameliorates STING-driven inflammatory diseases.
    Article Snippet: 1Drug Discovery and Design Center, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; 2University of Chinese Academy of Sciences, Beijing 100049, China; 3School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023, China; 4School of Pharmaceutical Science and Technology, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China; 5Shanghai Institute for Advanced Immunochemical Studies, and School of Life Science and Technology, Shanghai Tech University, Shanghai 200031, China; 6School of Physical Science and Technology, ShanghaiTech University, Shanghai 200031, China; 7State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210023, China

    Article Title: MyD88 signalling in B cells and antibody responses during Oropouche virus-induced neurological disease in mice
    Article Snippet: Plates were then washed again five times with washing buffer and incubated with 3,3',5,5'-tetramethylbenzidine (Sigma–Aldrich) for 30 min at room temperature in a dark chamber before reading at 650 nm using an Epoch Spectrophotometer (BioTek). .. IFN-α was quantified using a VeriKine Mouse Interferon-Alpha ELISA Kit (PBL Assay Science), and IFN-β was quantified using a Mouse IFN-Beta DuoSet ELISA Kit (R&D Systems). .. Levels of IFN-γ, IL-1β, IL-6, IL-10, IL-12, CXCL1, GM-CSF, and TNFα were determined using a Mouse Magnetic Luminex Assay Kit (R&D Systems).

    Article Title: TLR9 Monotherapy in Immune-Competent Mice Suppresses Orthotopic Prostate Tumor Development.
    Article Snippet: .. IFN-β levels in cell-culture media were measured using the Mouse IFN-beta DuoSet ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. .. GraphPad Prism 8.0 ( Boston, MA, USA) was used to perform unpaired, two-sided t-tests, or one- or two-way ANOVA analyses with post hoc tests for multiple comparisons (outlined in the figure legends).

    Article Title: The Toxoplasma Effector GRA4 Hijacks Host TBK1 to Oppositely Regulate Anti‐ T. Gondii Immunity and Tumor Immunotherapy
    Article Snippet: .. IFN‐β in cell supernatants and mice serum was measured with the Mouse IFN‐beta DuoSet ELISA kit (R&D SYSTEMS, Cat# DY8234‐05) following the assay procedure. .. Absorbance was detected at 450 nm by the Multiskan FC (Thermo Fisher Scientific).

    Article Title: The Toxoplasma Effector GRA4 Hijacks Host TBK1 to Oppositely Regulate Anti-T. Gondii Immunity and Tumor Immunotherapy.
    Article Snippet: .. Enzyme-Linked Immunosorbent Assay (ELISA): IFN-β in cell supernatants and mice serum was measured with the Mouse IFN-beta DuoSet ELISA kit (R&D SYSTEMS, Cat# DY8234-05) following the assay procedure. .. Absorbance was detected at 450 nm by the Multiskan FC (Thermo Fisher Scientific).

    Cell Culture:

    Article Title: T-cell derived extracellular vesicles prime macrophages for improved STING based cancer immunotherapy.
    Article Snippet: The concentration was determined from a standard curve made with IFN-α (IFNa2 PBL Assay Science) ranging from 2 to 500 U/mL. . Enzyme-linked Immunosorbent assay (ELISA) Protein levels of the cytokines CXCL-10 and IL-6 in the cell culture supernatants were determined using the following ELISA kits: CXCL-10 and IL-6 (R&D System) according to manufacturer’s instructions. .. IFN-beta in the cell culture supernatants was determined using the mouse IFN-beta DuoSet ELISA kit (R&D System) according to manufacturer’s instructions. .. IFNγ in the T-EVs were determined using the Human IFNγDuoSet ELISA kit (R&D System) according to manufacturer’s instruction.



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